Figure 2.

Effect of different priming on MC degranulation and cytokine release response to a secondary challenge. Degranulation of BMMCs primed with LPS (1mg/ml), curdlan (100mg/ml), IgE/Ag or RPMI was determined 6 days after cell recovery as percentages of released β-hexosaminidase in response to 30 min of stimulation with IgE/Ag (A) or ionomycin (B). BMMC primed with RPMI, LPS (1mg/ml), curdlan (100mg/ml) or IgE/Ag were subjected to a second stimulation with LPS (C–G), live C. Albicans (D–H), IgE/Ag (F–I) or curdlan (E–J). TNF-α (C–F) and IL-6 (G–J) levels were detected in culture supernatants after 24 hours. Data are expressed as mean +SD from n>4 experiments; statistical analysis were performed with one-way Anova with Dunnet correction (*p < 0,05 **p < 0,01; ***p < 0,001).