Skip to main content
. 2022 Feb 17;13:835348. doi: 10.3389/fimmu.2022.835348

Figure 4.

Figure 4

LPS-primed MCs differently activate the PI3K-AKT pathway. (A) Western Blot (WB) analysis of PI3K subunits and of phosphorylated p38 in differently primed-BMMC restimulated for 30 minutes with LPS or live C. Albicans (MOI=1). Numbers indicate the results of densitometric analyses calculated over endogenous protein expression. (B) Time-course WB analysis of phosphor Akt and phospho p38 in LPS primed-BMMC restimulated with LPS or live C. Albicans (MOI=1). Numbers indicate the results of densitometric analyses calculated over endogenous protein expression normalized versus t=0. (C) Mean + SD of densitometric analysis of n=3 independent experiments are reported. Statistical analyses were performed with paired Student t-test (*=p<0,05). NF-kB p65 (D) and p50 (E) activity in LPS primed BMMC was determined after 30 minutes form addition of the second stimuli. Results were expressed as fold percentages over control (RPMI). Mean + SD of n=3 independent experiments are reported. Statistical analysis were performed with one-way Anova with Dunnet correction (*p < 0,05 **p < 0,01; ***p < 0,001).