Table 1.

Effect of maternal immune activation on HuSA specific IgM and IgY titers during 4 weeks after i.t. immunization of offspring at 4 weeks of age.

	Isotype	Maternal treatment 1				Main effects ( P -value)
		PBS	HuSA	LPS	SEM	Time	Treatment	Time *Treatment4
Anti-HuSA	IgM2,3	3.2ab	3.0b	3.6a	0.2	<0.001	0.039	0.049
	IgY2,3	5.1b	5.6ab	6.9a	0.7	<0.001	0.030	0.024

¹Maternal treatment: i.t. immune activation of hens: 1 mg HuSA or 1 mg LPS in 0.5 ml PBS.

²Least squares means of i.t. immunization HuSA and HuSA+LPS combined.

³Least square means within a row lacking a common superscript (a, b or ab) are significantly different (P < 0.05).

⁴ Interacting variables are represented with an asterisk

(*).