Table 2.
Effect of dietary β-glucan on HuSA specific IgM and IgY titers in offspring after primary i.t. immunization with HuSA at 4 weeks of age.
| Isotype | PBS | HuSA | LPS | SEM | Main effects (P-value)1, 2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | β-Glucan | Control | β-Glucan | Control | β-Glucan | Time | Treatment 1 | Treatment 2 | Treatment 1 *Treatment 2 | Time *Treatment 1 | Time *treatment 25 | |||
| Anti-HuSA | IgM3,4 | 4.00 | 4.20 | 3.60 | 4.30 | 3.70 | 3.60 | 0.20 | <0.001 | 0.228 | 0.206 | 0.351 | 0.870 | 0.345 |
| IgY3,4 | 5.40ab | 6.21ab | 4.12b | 7.13a | 4.72ab | 4.80ab | 0.60 | <0.001 | 0.193 | 0.011 | 0.049 | 0.500 | 0.005 | |
1
Treatment 1: i.t. immune activation of mother hens: 1 mg HuSA and 1 mg LPS in 0.5 ml PBS.
2
Treatment 2: Dietary intervention with or without β-glucan enrichment in offspring; n = 5 pens per group.
3
Least squares means.
4
Least square means within a row lacking a common superscript (a, b or ab) are significantly different (P < 0.05).
5
Interacting variables are represented with an asterisk
(*).