TABLE 1.

Primer sequences for real time PCR.

Gene	Forward primer	Reverse primer	Product size (bp)
GAPDH	TGACTCTACCCACGGCAAGTTCAA	ACGACATACTCAGCACCAGCATCA	141
ACE	GTGTTGTGGAACGAATACGC	CCTTCTTTATGATCCGCTTGA	187
AT1-R	CTCAAGCCTGTCTACGAAAATGAG	GTGAATGGTCCTTTGGTCGT	188
NOX2	CAAGATGGAGGTGGGACAGT	GCTTATCACAGCCACAAGCA	170
TNF-α	GCCGATTTGCCACTTCATAC	AAGTAGACCTGCCCGGACTC	209
IL-6	GCCTATTGAAAATCTGCTCTGG	GGAAGTTGGGGTAGGAAGGA	160
IL-1β	AGCAACGACAAAATCCCT GT	GAAGACAAACCGCTTTTCCA	209
IL-10	GTTGCCAAGCCTTGTCAGAAA	TTTCTGGGCCATGGTTCTCT	120
AT2-R	ACCTTTTGAACATGGTGCTTTG	TTTCCTATGCCAGTGTGCAG	160
Mas-R	TGTGGGTGGCTTTCGATT	CCCGTCACATATGGAAGCAT	159

ACE, angiotensin converting enzyme 1; AT1-R, angiotensin II type 1 receptor; NOX2, NADPH oxidase 2; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; IL-1β, interleukin-1β; IL-10, interleukin-10; AT2-R, angiotensin II type 2 receptor; and Mas-R, angiotensin-(1–7) receptor.