FIGURE 1.

In vivo development of human brain organoids in mouse brain. (A) Experiment procedure for premature organoid generation and injection into mouse corpus striatum. (B) IVD‐organoids were immunostained for GFP and DAPI. Left, coronal section showing IVD‐organoids survived in mouse corpus striatum. Scale bar, 1000 μm. Middle, enlarged image showing the ventricle zone‐like structure. Scale bars, 50 μm. Right, enlarged image showing the choroid plexus epithelium‐like structure. Scale bar, 100 μm. (C) Comparation of grafted cells (left) and IVD‐organoids (right). Scale bars, 500 μm. (D) Immunostaining of MAP2 in IVD‐organoids. Scale bars, 20 μm. (E) Immunostaining of GFAP and STEM121 in IVD‐organoids. Scale bars, 50 μm and 10 μm (high‐magnification images). (F) Immunostaining of IBA1 in IVD‐organoids. Scale bars, 50 μm. (G) Immunostaining of vascular marker CD31 in IVD‐organoids. Scale bars, 50 μm