Figure 1.

Sat production by EC071. TCA-treated LB culture supernatants of EC071, DAEC EC233/93 (positive control) and Shigella flexneri M90T (negative control) were analyzed by 10% SDS-PAGE. (A) Silver nitrate-stained gel; (B) Immunoblotting with anti-Sat (1:500) and peroxidase-conjugated goat anti-rabbit IgG (1:10,000). Ladder: Precision Plus Protein Kaleidoscope Prestained Protein Standard (BioRad, USA).