Table 1.

Genetic constructions obtained in this study.

Strain	Description	Sat Production
EC071::pCF2	EC071 sat mutant	Absent
EC071::pCF2 (pCF3)	EC071 sat mutant complemented with sat minimal clone	Active serine protease
EC071::pCF2 (pCF4)	EC071 sat mutant complemented with sat site-directed mutant	Inactive serine protease
DH5α (pCF3)	DH5α harboring sat minimal clone	Active serine protease
DH5α (pCF4)	DH5α harboring sat site-directed mutant	Inactive serine protease
MG1655 (pCF3)	MG1655 harboring sat minimal clone	Active serine protease
MG1655 (pCF4)	MG1655 harboring sat site-directed mutant	Inactive serine protease