TABLE 8.

Primer pairs used for phylogenetic analysis of human microsporidia

Primer pair	Primer designation	Species amplified	Target gene	Approximate amplicon size (bp)	Reference(s)
5′-CACCAGGTTGATTCTGCCTGAC-3′	V1	E. bieneusi, E. intestinalis, E. cuniculi, E. hellem, other species	SSU rRNA	1,200	363, 381, 395, 396
5′-GGTTACCTTGTTACGACTT-3′	1492
5′-CACCAGGTTGATTCTGCC-3′	18f	E. bieneusi, E. intestinalis, E. cuniculi, E. hellem, other species	SSU rRNA	1,300	10
5′-TTATGATCCTGCTAATGGTTC-3′	1537r
5′-CACCAGGTTGATTCTGCCTGA-3′	Micro-F	E. intestinalis, E. cuniculi, E. hellem, other species	SSU rRNA	1,300	359, 360
5′-TTATGATCCTGCTAATGGTTCTCCAAC-3′	Micro-R
5′-CACCAGGTTGATTCTGCCTGA-3′	Micro-F	E. bieneusi	SSU rRNA	1,200	361
5′-CCAACTGAAACCTTGTTACGACTT-3′	1492N4
5′-CACCAGGTTGATTCTGCCTGACG-3′		E. bieneusi, E. intestinalis, E. cuniculi, E. hellem, other species	SSU rRNA	1,300	303
5′-TTATGATCCTGCTAATGGTTCTCC-3′
5′-GTGCCAGC(C/A)GCCGCGG-3′	530f	E. bieneusi, E. intestinalis, E. cuniculi, E. hellem, V. cornae, other species	SSU rRNA, ISRa, LSU rRNA	1,550, 1,350, 1,350, 1,350, 1,300b	108, 364, 381, 396
5′-GGTCCGTGTTTCAAGACGG-3′	580r
5′-TGCAGTTAAAATGTCCGTAGT-3′	int580f	E. intestinalis, E. cuniculi, E. hellem, other species	SSU rRNA, ISR, LSU rRNA	1,000, 1,000, 1,000b	108, 303
5′-TTTCACTCGCCGCTACTCAG-3′	int580r

^aISR, intergenic spacer region. 

^bSizes given for listed species respectively.