Skip to main content
. 2022 Feb 17;9:743124. doi: 10.3389/fcell.2021.743124

FIGURE 4.

FIGURE 4

v-ATPase is compromised in KD-HPS6 human umbilical vein endothelial cells (HUVECs). Pharmacological inhibition of the v-ATPase caused the Weibel–Palade bodies (WPBs) to lose their elongated shapes. DMSO or v-ATPase inhibitor, Baf A1 (200 nM), was administered 1 h before cell fixation by 4% PFA (n = 20 per group). (A, B) Immunofluorescence images of DMSO-treated (A) and Baf A1-treated cells labeled against vWF (red) and nucleus (DAPI, blue). Scale bar, 10 μm. (C) Feret’s diameter of WPBs at each group of HUVECs was analyzed quantitatively (DMSO: 502 WPBs, gray; Baf A1: 300 WPBs, black). (D–I) siRNA-mediated HPS6 knockdown suppressed the expression of ATP6V0D1, ATP6V1H, and ATP6V1B2, three subunits of v-ATPase (n = 8 per group, **p < 0.01,***p < 0.001). Data were expressed as mean ± SEM. Three independent experiments were performed.