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Restriction enzyme digestion screening for UL97 mutations related to ganciclovir resistance
| Enzyme | UL97 Codon | Fragment size (bp)a
|
|
|---|---|---|---|
| Wild-type sequence | Mutant sequence | ||
| NlaIII | 460 | 198, 168, 126, 9 | 324, 168, 9 |
| AluI | 520 | 295, 189, 17 | 268, 189, 27, 17 |
| HhaI | 594 | 50, 38, 18, 12 | 62, 38, 18 |
| MseI | 595 | 76, 42 | 46, 42, 30 |
| TaqI | 595 | 99, 19 | 71, 28, 19 |
Underlined fragments are normally seen in polyacrylamide gels after ethidium bromide staining.
