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. 2022 Feb 23;15(3):dmm049133. doi: 10.1242/dmm.049133

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Behavioral phenotype of mao mutants. (A) mao^−/− larvae were hypoactive during diurnal and nocturnal (gray shaded area) locomotor activity tracking at 6 dpf. n=16 for mao^+/+, n=16 for mao^+/− and n=15 for mao^−/−. (B) Larvae at 6 dpf were exposed to alternating 2 min periods of light and darkness (gray shaded areas), with three periods of darkness in total. n=15 mao^+/+ and n=16 for mao^+/− and mao^−/−. (C-E) Analysis of the larval response to sudden darkness [first (C), second (D) and third (E) flash response] in 1 s bins. Gray shaded areas indicate periods of darkness. (F) Mean values for maximum velocity during ten acoustic/vibrational stimuli with a 20 s interstimulus interval (ISI). n=15 for mao^+/+, n=16 for mao^+/− and n=12 for mao^−/−. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. ^#P<0.05 (mao^+/− versus mao^+/+ and mao^−/−), *P<0.05 (mao^+/+ and mao^+/− versus mao^−/−), ^§P<0.05 (mao^+/+ versus mao^−/−), ^€P<0.05 (mao^+/− versus mao^−/−), ^‡P<0.05 (mao^+/− versus mao^+/+).