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. 2022 Feb 23;15(3):dmm049133. doi: 10.1242/dmm.049133

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — The serotonergic system of mao mutants. (A-C) Ventral views of whole-mount 10 dpf mao^+/+ (A), mao^+/− (B) and mao^−/− (C) larval brains, anterior to the left, processed for serotonin (5-HT) immunostaining. Arrow indicates ectopic cells in mao^−/− brain. n=5 for each genotype. (D-F) Ventral views of whole-mount 10 dpf mao^+/+ (D), mao^+/− (E) and mao^−/− (F) larval brains, anterior to the left, processed for tph1a RNA in situ hybridization (ISH). (G-I) Bar charts showing results of 5-HT-immunoreactive cell counting in larval brains [paraventricular organ anterior part (PVOa; G), paraventricular organ intermediate part (PVOi; H) and paraventricular organ posterior part (PVOp; I)] of the indicated genotype at 10 dpf. (J,K) Bar charts showing results of reverse transcriptase-quantitative PCR (RT-qPCR) analysis of tph1a (J) and mao (K) in larvae of the indicated genotype at 10 dpf. Data are mean±s.e.m. One-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *P<0.05, **P<0.01, ***P<0.001. Scale bars: 75 μm.