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. 2022 Feb 23;15(3):dmm049133. doi: 10.1242/dmm.049133

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — mao^−/− larvae exhibit altered expression of developmental markers. (A,B) Ventral views of whole-mount 10 dpf mao^+/+ (A) and mao^−/− (B) larval brains, anterior to the left, processed for notch1a RNA ISH. n=4 for each genotype. (C-F) Bar charts showing results from RT-qPCR analysis of notch1a (C), neurod1 (D), apoeb (E) and gfap (F) in larvae of the indicated genotype at 10 dpf. n=4 for each genotype. Data are mean±s.e.m. One-way ANOVA followed by Tukey's multiple comparisons test for statistical analysis. *P<0.05, **P<0.01. Scale bar: 75 μm.