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. 2022 Feb 23;15(3):dmm049133. doi: 10.1242/dmm.049133

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Social preference of mao^+/− adult fish is impaired. (A) Representation of the apparatus used to study the social behavior of adult mao^+/+ and mao^+/− fish and traces of the swimming pattern from both genotypes. Fish behavior was evaluated during a 10 min video recording session in a home-made social interaction apparatus. The apparatus is physically divided into three chambers and digital zones (distal, social and non-social), which were established with EthoVision XT 13 software. n=8 for each genotype. (B-E) Bar charts showing results on the time spent in each zone [social (B), non-social (C), distal (D) and total (E)]. Compared to their siblings, mao^+/− adult fish did not differ in the time spent closer to the social stimulus, spent less time in the non-social zone and more time in the distal zone. When the time spent in the distal zone and non-social zones was summed, the mao^+/+ fish show a preference for the area closer to the social stimulus, whereas when the same comparison is done with their mao^+/− siblings, no preference is detected. Data are mean±s.e.m. Unpaired, two-tailed Student's t-test was used for statistical analysis. *P<0.05.