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. 2022 Feb 23;15(3):dmm049133. doi: 10.1242/dmm.049133

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — Gene expression in adult mao^+/+ and mao^+/− fish. (A-F) Bar charts showing results from RT-qPCR analysis of shank3b (A), mecp2 (B), hrh3 (C), mao (D), vmat2 (E) and serta (F) in adult brains of the indicated genotype. A significant decrease in the expression of mao was detected in mao^+/− fish compared to mao^+/+ siblings. A significant upregulation in the expression of vmat2 was detected in mao^+/− fish compared to mao^+/+ siblings. (G,H) Dorsal views of whole-mount adult mao^+/+ (G) and mao^+/− (H) brains, anterior to the top, processed for serta RNA ISH. serta-positive neurons expressed in the ventral posterior tuberculum. (I,J) Ventral views of whole-mount adult mao^+/+ (I) and mao^+/− (J) brains, anterior to the right, processed for serta RNA ISH. serta-positive neurons expressed in the raphe nuclei and hindbrain. Data are mean±s.e.m. Unpaired, two-tailed Student's t-test was used for statistical analysis. *P<0.05, ***P<0.001. Scale bar: 75 μm.