Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 26;29:10732748221081369. doi: 10.1177/10732748221081369

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 4. — PER2 knockdown promotes RKO cell migration and activates the EMT process. (A) Detect the number of cell migration in the shPER2 and shControl groups by Transwell. (B) The number of migratory cells was counted by ImageJ, scale bar 100 µm (*** P <.001). (C) Determine the cell migration after PER2 knockdown after 48 and 72h by Wound-healing assay. (D) The percentage of wound area closure was analyzed by ImageJ, scale bar 200 µm, shown in the lower panel (**** P <.0001). (E) Mitomycin was added to remove the effect of proliferation on migration. (F) The percentage of wound area closure. (G) The expression of EMT-associated markers was detected by western blotting. EMT, epithelial-mesenchymal transformation.