Table 2.
Representative commercially available anti-ASPH antibodies with labyrinthin antigen overlap.
| ANTIBODY | ASPH a.a. target | LABYRINTHIN a.a. overlap | ANTIBODY VALIDATION∗ |
|---|---|---|---|
|
Abnova monoclonal H00000444-M09 |
175–284 | 117–226 | Sandwich ELISA (rASPH) |
| polyclonal H00000444–B02P |
35-107 (Junctate 1–55) | 1–49 | HEK293T: WB (25 kDa) |
|
Antibodies-Online polyclonal ABIN950540 |
301–331 | 243-253, 255 | Stomach tissue, IHC A549 cells, WB (85 kDa) |
|
Bethyl Laboratories polyclonal A305-343A |
708–758 | none | HeLa, WB (180 kDa; minor 165, 65 kDa) HEK293T, WB (minor 180, 165, 65 kDa) |
| polyclonal A305-342A-M |
300–350 | 242–255 | HeLa, WB (115 kDa) HEK293T, WB (minor 115 kDa) |
| Bioss Antibodies bs-12137R | 301–400 | 243-253, 255 | ELISA, IHC, IF applications mentioned |
|
Creative Biolabs polyclonal MOR-0269 |
250–350 | 192–255 | WB, IHC-P, IF, FC applications mentioned |
| rHuman IgG1 MHH-78 |
1–758 | 1–255 | Binds to ASPH in HEK 293 cells mentioned |
| polyclonal MOR-0269 |
250–350 | 192–255 | WB, IHC-P, IF, FC applications mentioned |
|
Invitrogen polyclonal PA5-65929 |
506–600 | none | HeLa, IF: localization to ER |
| polyclonal PA5-63702 |
81–176 | 21–118 | Cerebral cortex tissue, IHC: cytoplasmic staining in astrocytes |
| polyclonal PA5-78827 |
726–758 | none | Mammary cancer tissue, IHC Rat brain tissue, rat liver tissue, HeLa, HEPG2, WB (all ∼100 kDa) |
| polyclonal PA5-97476 |
75–270 | 16–212 | HeLa, IF: cytoplasmic Adrenal gland tissue, brain tissue, IHC A549 cells, HepG2 cells, mouse kidney tissue lysates, WB (all 86 kDa) |
| polyclonal PA5-40954 |
121–170 | 63–112 | Small intestine, myenteric plexus tissue, IHC Adult heart tissue, IF: cytoplasmic, some peripheral punctate ACHN cells, WB (35 kDa) |
| polyclonal PA5-43688 |
88-107 (Junctate 66–93) | 24–49 | Kidney, IHC HepG2 cell, WB (21 kDa) |
|
Proteintech Group polyclonal 14116-1-AP |
1–84 | 1–26 | Kidney, IHC Brain, WB (30 kDa) A549, WB (25 kDa) |
List information obtained from Labome and Antibodypedia. All cells and tissues are human-derived. Unless otherwise specified, all polyclonal and monoclonal antibodies are from rabbit and mouse, respectively.
∗Abbreviations not listed elsewhere: IF, Immunofluorescence; WB, western Immunoblot; ( ), approximate molecular weight; FC, flow cytometry; HEK293T, embryonic kidney; HeLa, cervical cancer cells, ACHN, renal adenocarcinoma; HepG2, liver hepatocellular carcinoma.