Figure 3.

Overview of ESP-based Sliding Lid for Immobilized Droplet Extraction (SLIDE).A, schematic of SLIDE technique using EXTRACTMAN device (Gilson, Inc). The exclusion phase operates by repeated cycles of lifting of magnetic beads loaded with immunoprecipitate out of the wash solution. The extracted drops are held against a hydrophobic surface with minimum aqueous volume and surface tension before re-elution in a fresh aqueous solution. B, an overview of the SLIDE protocol, showing the configuration and dimensions of this specific device. ESP, Exclusion-Based Sample Preparation.