Figure 6.

Effects of NHFs conditioned medium on NHMs melanogenesis

(A) Western blot analysis of tyrosinase protein expression in NHMs after treatments with NHFs medium (see Table 3 for treatments). GAPDH was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case).

(B) Analysis of tyrosinase activity on NHMs after treatments. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01) and R 1/4 (^$p < 0.05, ^$$p < 0.01), respectively.

(C) Melanin content evaluation in NHMs after treatments. Results are presented as the mean ± SD of three independent experiments and are expressed as ratio of μg melanin/mg protein (∗p < 0.05, ∗∗p < 0.01 versus Ctr; ^$p < 0.05, ^$$p < 0.01 versus R 1/4).