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ASC and ASCb structure comparison by NMR.A, ribbon diagram of the polypeptide backbone structure of ASC (10) and ASCb (model created with the program Chimera (23) based on the PDB structure of ASC (10)). B, 2D-[1H-15N]-HSQC (NMR protein fingerprint of ASC and ASCb showing almost complete signal overlap). C, combined-average amide 1H and 15N chemical shift differences between ASC and ASCb as described in the Experimental procedures section (solid line: 1 SD; dashed line: 2 SD).
