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. 2022 Feb 17;10:790410. doi: 10.3389/fcell.2022.790410

FIGURE 3.

FIGURE 3

2D migratory characteristics on ECM binding proteins. (A) Phase contrast images of MDA-P, MDA-BR, and MDA-BO cells on Collagen I, Fibronectin, or PDL coated substrates. Scale bars represent 100 μm (in zoomed out images) or 50 μm (in zoomed insets). (B) Speed tracked for 360 min at 5-min intervals. Groups with # on top are different from corresponding cell type on Fibronectin substrate with p < 0.001. Groups with $ on top are different from corresponding cell type on Collagen I substrate with p < 0.001. (C) Diffusion coefficient calculated from mean squared displacement (MSD) vs. time data (over 300 min), as described in the Methods section. Also shown is mean MSD vs. time interval plots for migration on (D) Collagen I, (E) Fibronectin, and (F) PDL. In panel B, box and whisker plots represent the minimum and maximum data points, the median, and the interquartile range; each column of data is from N > 37 cells pooled from at least 3 independent experiments. Panel B data was tested for normality using the D’Agostino and Pearson test and found to be not normally distributed. A Kruskal Wallis test and a Dunn’s multiple comparison test was performed to determine significance.