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. 2022 Jan 30;101(4):101753. doi: 10.1016/j.psj.2022.101753

Table 1.

Quantitative real-time RT-PCR primer and probe sequences.

Target Primer and probe sequence Accession no.2
28s Forward
Reverse
Probe
GGCGAAGCCAGAGGAAACT
GACGACCGATTTGCACGTC
15´-(FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA)-3´
X59733
IL6 Forward
Reverse
Probe
GCTCGCCGGCTTCGA
GGTAGGTCTGAAAGGCGAACAG
5´-(FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(TAMRA)-3´
AJ250838
IL10 Forward
Reverse
Probe
CATGCTGCTGGGCCTGAA
CGTCTCCTTGATCTG CTTGATG
5´-(FAM)-CGACGATTCGGCGCTGTCACC-(TAMRA)-3´
AJ621614
IFN- γ Forward
Reverse
Probe
GTGAAGAAGGTGAAAGATATCATGGA
GCTTTGCGCT GGATTCTCA
5´-(FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA)-3´
Y07922
1

FAM Fluorescent reporter dye 5-carboxyfluorescein; TAMRA quencher N, N, N, N´-tetramethyl-6-carboxyrhodamine

2

Genomic DNA sequence.