Table 1.
Target | Primer and probe sequence | Accession no.2 | |
---|---|---|---|
28s | Forward Reverse Probe |
GGCGAAGCCAGAGGAAACT GACGACCGATTTGCACGTC 15´-(FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA)-3´ |
X59733 |
IL6 | Forward Reverse Probe |
GCTCGCCGGCTTCGA GGTAGGTCTGAAAGGCGAACAG 5´-(FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(TAMRA)-3´ |
AJ250838 |
IL10 | Forward Reverse Probe |
CATGCTGCTGGGCCTGAA CGTCTCCTTGATCTG CTTGATG 5´-(FAM)-CGACGATTCGGCGCTGTCACC-(TAMRA)-3´ |
AJ621614 |
IFN- γ | Forward Reverse Probe |
GTGAAGAAGGTGAAAGATATCATGGA GCTTTGCGCT GGATTCTCA 5´-(FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA)-3´ |
Y07922 |
FAM Fluorescent reporter dye 5-carboxyfluorescein; TAMRA quencher N, N, N, N´-tetramethyl-6-carboxyrhodamine
Genomic DNA sequence.