Table 6.
Average ELISA titers of control (non-vaccinated) and FMG in ovo vaccinated1 Hy-Line W-36 layer chickens at 14, 21, and 28 d of age.
| D 14 | D 21 | D 28 | |
|---|---|---|---|
| Control | 147.2 | 218.1 | 155.1 |
| FMG in ovo | 288.1 | 416.1 | 660.6 |
| Pooled SEM | 18.8 | 23.7 | 45.7 |
| Probability | |||
| Trt | 0.0062 | 0.0095 | <0.0001 |
| Sex2 | 0.4870 | 0.2729 | 0.1306 |
| Trt × Sex | 0.0695 | 0.0607 | 0.2660 |
Chickens were vaccinated with Poulvac Myco F (Zoetis) live attenuated strain F Mycoplasma gallisepticum (FMG) vaccine at 18 days of incubation with a 10−6 dilution of the vaccine in a 50 µL volume. The vaccine was resuspended and diluted in Poulvac Marek's diluent (Zoetis, Exton, PA).
Sex was determined by necropsy of the deceased birds and evaluation of the internal reproductive organs.