Fig. 2.

Characterizations of exosomes derived from hADMSCs and hADMSC-SCs. (A) Schematic of the protocol for exosome isolation from cell culture media. (B) Size distribution of uExo and dExo was obtained from NTA test, inset showing representative exosome images captured from the NTA video frames. (C) AFM images of uExo and dExo (scale bar 250 nm). (D) TEM images of uExo and dExo (scale bar 100 nm). (E) Proteins from each centrifugal rate (1k × g : 1000 × g, 10k × g:10,000 × g, and 100k × g: 100,000 × g) were analyzed by western blot for the presence of exosome-associated proteins CD9, CD63, TSG101, and Alix.