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. 2021 Dec 14;14:61–75. doi: 10.1016/j.bioactmat.2021.11.022

Fig. 6.

Fig. 6

Effects of uExo and dExo on the growth of SNs derived from hiPSCs. (A) Schematic of the differentiation protocol of hiPSCs into SNs and further hiPSC-SN neurospheres. (B) Expression levels of ISL1, PRPH, BRN3A, OCT4, NTRK1, SCN9A, SCN10A, SCN11A, P2RX3, and TRPV1 for SNs were evaluated by RT-PCR. (n = 3). (C) IF staining images of hiPSC-SNs. hiPSC-SNs were positive for β-3 tubulin, TRPV1, and DRAQ5. Scale bar 50 μm. (D) IF staining images of hiPSC-SN neurospheres with β-3 tubulin, TRPV1, and DAPI. Scale bar 100 μm. (E) Representative IF images of axons from neurospheres after being encapsulated within Matrigel and treated with PBS, uExo, or dExo. Scale bar 500 μm. (F) Representative optical microscopic images of encapsulated neurospheres treated with PBS, uExo, or dExo for 6 days. Scale bar 500 μm (G) Semi-quantitative analysis of axon length of encapsulated neurospheres after treatment with uExo or dExo for 0, 2, 4, and 6 days (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.