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. 2022 Feb 2;298(3):101669. doi: 10.1016/j.jbc.2022.101669

Table 1.

Kinetic properties of the NAMPT proteins

NAMPT protein Km (μM)
Vmax (nmol/min/mg)
Vmax/Km
Ki (μM)
Nama PRPPb PRPP Nam PRPP
Wildtype <0.1 0.46 ± 0.13 123 ± 2 586 103 ± 22 52 ± 13
α-Loop double mutant K(68/71)S <0.1 0.28 ± 0.08 122 ± 14 381 93 ± 19 93 ± 24
α-Loop triple mutant K(68>73)A <0.1 0.29 ± 0.05 108 ± 23 512 69 ± 19 104 ± 20
β-Loop double mutant K(42/48)A <0.1 0.73 ± 0.14 148 ± 13 487 166 ± 41 103 ± 23
β-Loop full mutant K/R(42>51)A <0.1 0.89 ± 0.14 110 ± 11 177 183 ± 52 905 ± 225
β-Loop deleted mutant ΔK(42>51)c <0.1 0.12 ± 0.01 0.6 ± 0.02 5 ND ND
172 780 ± 40 1.3 ± 0.05 0.0017 ND ND

Abbreviation: ND, not detectable.

a

At 0.1 mM PRPP, 1 mM ATP.

b

At 5 μM Nam, 1 mM ATP.

c

Upper row shows kinetic parameters for the first phase (i.e., Km1, Vmax1), lower row for the second stage of kinetics (Km2, Vmax2).