Table 2.

Kinetic and equilibrium parameters of the interactions between surface-blocked TLR4 and soluble NAMPT variants

TLR4-NAMPT complex	kass (M−1s−1)	kdiss (s−1)	KD (M)
TLR4-wild type	(2.8 ± 0.3) 105	0.005 ± 0.001	(1.78 ± 0.39) 10−8
TLR4–α-loop double mutant K(68/71)S	(4.8 ± 0.7) 105	0.01 ± 0.004	(2.08 ± 0.89) 10−8
TLR4–α-loop triple mutant K(68>73)A	(3.1 ± 0.7) 103	0.004 ± 0.002	(1.28 ± 0.70) 10−6
TLR4–β-loop double mutant K(42/48)A	(7.8 ± 0.5) 105	0.029 ± 0.003	(3.71 ± 0.46) 10−8
TLR4–β-loop full mutant K/R(42>51)A	(1.3 ± 0.7) 105	0.085 ± 0.023	(6.30 ± 3.50) 10−7
TLR4–β-loop deleted mutant Δ(42>51)	(3.2 ± 0.4) 105	0.07 ± 0.02	(2.18 ± 0.68) 10−7