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. 2022 Feb 17;12:816238. doi: 10.3389/fcimb.2022.816238

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Copyright © 2022 Wang, Zhang, Zhou, Li, Chen, Liu, Liu, Ding, Liang, Zhu, Zhang, Xin, Zhang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimal primer selection. Products amplified by different reverse transcription recombinase-aided amplification primers were analyzed by 1.5% agarose gel electrophoresis to select the optimal primer pairs. M is a 2,000-bp DNA marker. The primers and templates used in lanes 1–8 are as follows: 1, HCV-RAA-F1/R1 + HCV; 2, HCV-RAA-F1/R1 + ddH₂O; 3, HCV-RAA-F1/R2 + HCV; 4, HCV-RAA-F1/R2 + ddH₂O; 5, HCV-RAA-F2/R1 + HCV; 6, HCV-RAA-F2/R1 + ddH₂O; 7, HCV-RAA-F2/R2 + HCV; 8, HCV-RAA-F2/R2 + ddH₂O.