Figure 2.

Optimal primer selection. Products amplified by different reverse transcription recombinase-aided amplification primers were analyzed by 1.5% agarose gel electrophoresis to select the optimal primer pairs. M is a 2,000-bp DNA marker. The primers and templates used in lanes 1–8 are as follows: 1, HCV-RAA-F1/R1 + HCV; 2, HCV-RAA-F1/R1 + ddH₂O; 3, HCV-RAA-F1/R2 + HCV; 4, HCV-RAA-F1/R2 + ddH₂O; 5, HCV-RAA-F2/R1 + HCV; 6, HCV-RAA-F2/R1 + ddH₂O; 7, HCV-RAA-F2/R2 + HCV; 8, HCV-RAA-F2/R2 + ddH₂O.