Skip to main content
. 2022 Feb 9;298(3):101701. doi: 10.1016/j.jbc.2022.101701

Figure 1.

Figure 1

Ablation of the MuvB components Lin54 and Lin52 causes exit from pluripotency in ESCs.A, schematic overview of the generation for Lin54 conditional inactivation via CRISPR/Cas9-mediated gene targeting in ESCs. B, Western blot revealing the loss of Lin54 protein in ESCs after Cre recombinase expression. Tubulin was used as a loading control. C, morphological images of ESC colonies of indicated genotypes. ESC colonies were photographed at day 7 after seeding single-cell suspensions on MEF. Images were taken at 100× magnification. Red circles represent the borders of ESC colonies. D, shown were alkaline phosphatase staining images of indicated ESC colonies. Scale bar, 100 μm. E and F, Western blot demonstrating the expression levels of Lin54, Lin52, M2, Sox2, Oct4 and Nanog in ESCs of indicated genotypes. Tubulin served as a loading control. Results shown in (B–F) are representative of three independent experiments performed with three different clones of each genotype. ESC, embryonic stem cell; HR, homologous recombination; MuvB, multivulva class B; WT, wild-type.