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. 2022 Jan 17;23(3):e53191. doi: 10.15252/embr.202153191

Figure EV1. ZFP207 is dispensable in mouse ESCs maintained in ground state pluripotency.

Figure EV1

  • A, B
    (A) AP staining of shScr and Zfp207‐depleted (sh1 and sh2) mouse ESCs cultured in 2iL. Scale bars, 20 µM. (B) Percentage of fully differentiated (FD), partially differentiated (PD) and undifferentiated (UN) ESC colonies in shScr, sh1 and sh2 cultured in 2iL.
  • C, D
    (C) Western blot of ZFP207, OCT4, NANOG, and SOX2 and (D) RT‐qPCR analysis of Zfp207, Oct4, Nanog, and Sox2 in shScr, sh1, and sh2 ESCs cultured in 2iL; data are relative to shScr.
  • E
    Immunofluorescence analysis of SSEA1 in shScr, sh1, and sh2 ESCs cultured in 2iL. DAPI was used as the nuclear marker. Scale bars, 20 μm.

Data information: Data are presented as mean ± SEM or representative images of n ≥ 3 independent biological experiments. **P < 0.01, n.s = no significant difference (shScr versus sh1 or sh2). B: unpaired Student’s t‐test; D: Ordinary one‐way ANOVA.

Source data are available online for this figure.