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. 2022 Jan 17;23(3):e53191. doi: 10.15252/embr.202153191

Figure EV2. Zfp207 knockdown ESCs cannot generate NPCs.

Figure EV2

  • A
    Schematic diagram for neural progenitor cells (NPC) generation. Retinoic acid (RA) was added after four days of EB culture.
  • B, C
    (B) Representative bright‐field images (10×) and (C) quantification of NPCs on day 6 of differentiation. Scale bars, 200 μm.
  • D
    RT‐qPCR of Zfp207 and neural‐associated markers in shScr, sh1 and sh2 along the course of neural progenitor (NPC) differentiation. mRNA levels are relative to shScr at day 0.

Data information: Data are presented as mean ± SEM or representative images of n ≥ 3 independent biological experiments. *P < 0.05, **P < 0.01, ****P < 0.0001. C: Ordinary one‐way ANOVA; D: two‐way ANOVA.