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. 2022 Jan 17;23(3):e53191. doi: 10.15252/embr.202153191

Figure 3. ZFP207 is essential for neural cell fate specification.

Figure 3

  • A
    Schematic depiction for the neuroectodermal‐directed differentiation. DMEM/F12 supplemented with N2B27 and retinoic acid (RA) was added after two days of EB culture.
  • B
    Immunostaining of NESTIN (green) and TUJ1 (red) of neural progenitors generated from shScr on day 8 and 10 of the neuroectodermal differentiation. Nuclei were counterstained with DAPI. Scale bar, 20 μM.
  • C–E
    (C) TUNEL (green), (D) CASPASE 3 (red) staining and (E) quantification of the signal in shScr, sh1, and sh2 at day 5 of neuroectodermal differentiation. Nuclei were counterstained with DAPI. Scale bar, 20 µM.
  • F
    Flow cytometric profile and quantification of BrdU incorporation at day 4 of neuroectodermal differentiation.
  • G
    RT‐qPCR of Zfp207 and neural‐associated markers in shScr, sh1, and sh2 along the course of neural differentiation. mRNA levels are relative to shScr at day 0.

Data information: Data are presented as mean ± SEM or representative images of n ≥ 3 independent biological experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (shScr versus sh1 or sh2). E, F, and G: unpaired Student’s t‐test.