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. 2021 Dec 27;23(3):e54160. doi: 10.15252/embr.202154160

Figure EV4. Myosin VI ablation causes p53‐dependent cell cycle arrest.

Figure EV4

  • A–C
    Severe proliferation impairment in myosin VI‐depleted RPE1 cells is reproducible using a second siRNA oligo against myosin VI (KD#2). Impairment in cell proliferation was previously shown in rat p53‐proficient PC12 cells (Majewski et al, 2011). (A) Growth curve of hTERT‐RPE1 cells transfected with a second myosin VI siRNA oligo +/− p53 siRNA oligo. A representative plot of three independent experiments is shown. (B) Representative bright‐field images of cells treated with the indicated p53 and myosin VI siRNAs. Scale bar 200 nm. (C) IB analysis of control and myosin VI‐depleted hTERT‐RPE cells with anti‐myosin VI, anti‐p53 and anti‐p21 antibodies.
  • D–F
    Myosin VI depletion does not affect cell proliferation in p53‐null tumour cells. (D) Growth curve of hTERT‐RPE1, HeLa and Caco‐2 cells transfected with myosin VI siRNA measured by Incucyte. A representative plot of two independent experiments is shown. Data are reported as mean ± SD of three technical replicates. (E) Cell viability of the indicated cell lines measured by crystal violet staining. (F) IB analysis of the indicated cells lines, control and myosin VI‐depleted, with anti‐myosin VI, anti‐p53 and anti‐p21 antibodies. Anti‐GAPDH was used as loading control.

Source data are available online for this figure.