Primary antibodies | ||||
---|---|---|---|---|
Epitope | Species | Supplier | Identifier | Application |
53BP1 | Rabbit | Abcam | ab36823 | WB 1:1,000 |
Acetylated tubulin | Mouse | Sigma | T7451 | IF 1:1,000 |
Acetylated tubulin | Mouse | Sigma | 6‐11B‐1 | IF 1:1,000 |
Centrin1 | Mouse | Millipore | 04‐1624 | IF 1:1,000 |
Cep135 | Rabbit | Abcam | ab75005 | IF 1:1,000 |
Cep164 | Goat | Santa‐Cruz Biotechnology | sc‐240226 | IF 1:700 |
FBF1 | Rabbit | Sigma | HPA023677 | IF 1:50 |
FLAG | Mouse | Sigma | F3165 | WB 1:5,000 |
GAPDH | Mouse | Santa‐Cruz Biotechnology | Sc‐32233 | WB 1:5,000 |
GFP | Rabbit | Sigma | G1544 | WB 1:5,000 |
GFP | Mouse | Thermo Fisher Scientific | A‐11120 | PLA 1:2,000 |
GST | Rabbit | Generated in house | IP | |
LC3 | Rabbit | Cell Signaling | 12741 | WB 1:1,000 |
Myosin VI | Mouse | Sigma | MUD‐19 | WB 1:1,000 |
Myosin VI | Rabbit | Generated by EUROGENTEC S.A., purified by Cogentech | 1295 | IP |
Myosin VI | Rabbit | Generated by EUROGENTEC S.A., purified by Cogentech | 1296 | IP; WB 1:2,000; IF 1:400 |
ODF2 | Rabbit | Abcam | ab43840 | IF 1:200 |
OFD1 | Rabbit | Sigma | HPA031103 | IF 1:500; PLA 1:2,000 |
OFD1 | Rabbit | Kindly provided by Brunella Franco (TIGEM, Napoli) (Giorgio et al, 2007) | WB 1:100 | |
p21 | Rabbit | Cell Signaling | 2947 | WB 1:5,000 |
p53 (DO‐1) | Mouse | Cell Signaling | 18032 | WB 1:1,000 |
p62 | Rabbit | Enzo Life Sciences | BML_PW9860 | WB 1:1,000 |
PCM1 | Mouse | Sigma | SAB1406228 | IF 1:200 |
Pericentrin | Mouse | Abcam | 28144 | IF 1:200 |
IgG from rabbit serum | Sigma | I5006 | IP |