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. 2021 Dec 27;23(3):e54160. doi: 10.15252/embr.202154160
Primary antibodies
Epitope Species Supplier Identifier Application
53BP1 Rabbit Abcam ab36823 WB 1:1,000
Acetylated tubulin Mouse Sigma T7451 IF 1:1,000
Acetylated tubulin Mouse Sigma 6‐11B‐1 IF 1:1,000
Centrin1 Mouse Millipore 04‐1624 IF 1:1,000
Cep135 Rabbit Abcam ab75005 IF 1:1,000
Cep164 Goat Santa‐Cruz Biotechnology sc‐240226 IF 1:700
FBF1 Rabbit Sigma HPA023677 IF 1:50
FLAG Mouse Sigma F3165 WB 1:5,000
GAPDH Mouse Santa‐Cruz Biotechnology Sc‐32233 WB 1:5,000
GFP Rabbit Sigma G1544 WB 1:5,000
GFP Mouse Thermo Fisher Scientific A‐11120 PLA 1:2,000
GST Rabbit Generated in house IP
LC3 Rabbit Cell Signaling 12741 WB 1:1,000
Myosin VI Mouse Sigma MUD‐19 WB 1:1,000
Myosin VI Rabbit Generated by EUROGENTEC S.A., purified by Cogentech 1295 IP
Myosin VI Rabbit Generated by EUROGENTEC S.A., purified by Cogentech 1296 IP; WB 1:2,000; IF 1:400
ODF2 Rabbit Abcam ab43840 IF 1:200
OFD1 Rabbit Sigma HPA031103 IF 1:500; PLA 1:2,000
OFD1 Rabbit Kindly provided by Brunella Franco (TIGEM, Napoli) (Giorgio et al, 2007) WB 1:100
p21 Rabbit Cell Signaling 2947 WB 1:5,000
p53 (DO‐1) Mouse Cell Signaling 18032 WB 1:1,000
p62 Rabbit Enzo Life Sciences BML_PW9860 WB 1:1,000
PCM1 Mouse Sigma SAB1406228 IF 1:200
Pericentrin Mouse Abcam 28144 IF 1:200
IgG from rabbit serum Sigma I5006 IP