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. 2022 Feb 17;13:813849. doi: 10.3389/fmicb.2022.813849

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Copyright © 2022 Leopold, Abdelraouf, Nicolau, Agresta, Johnson, Teter, Dunne, Broadwell, van Belkum, Schechter, Sodergren and Weinstock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolomic profiling. Principal component analysis of non-targeted TripleTOF mass spectrometer (MS) data of mouse feces successfully grouped treatment from non-treatment mice. The scores plot for PC1 and PC3 of MS features in positive ion mode shows good separation of the antibiotic vs. non-antibiotic time points. Furthermore, clusters of post-antibiotic immunocompetent and post-antibiotic neutropenic features also show good separation from each other with immunocompetent clustering above and neutropenic below the x-axis.