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. 2022 Feb 25;119(9):e2026355119. doi: 10.1073/pnas.2026355119

Fig. 2.

Fig. 2.

The identification of gene-regulated seed vigor from the coexpression network of TFs and targeted genes. (A and B) The coexpression network of TFs and targeted genes both DE during Kasalath and Jigeng88 seed aging, respectively. Only TF–gene targets showing Pearson correlation > 0.7 or < −0.7 in expression were projected onto the network. TFs were plotted in different sizes according to the count of their targeted genes. Note that WRKY family TFs were shown as negative regulators for targeted gene expression. The genes in the figure are detailed in SI Appendix, Dataset S6. (C) The change of seed germination of WT, bZIP23 knockout (bzip23#TI, bzip23#19, and bzip23#20), and overexpression transgenic (35S:bZIP23#6 and 35S:bZIP23#9) plants during seed aging. bZIP23 mutations were mediated by both T-DNA insertion (bzip23#TI) and CRISPR/Cas9 (bzip23#19 and bzip23#20). (D) The change of seed germination of WT and bZIP42 CRISPR knockout (bzip42#10, bzip42#27, and bzip42#32) plants during aging. (E) The change of seed germination in WT, PER1A knockout (per1a#6 and per1a#9), and overexpressed (35S:PER1A#2 and 35S:PER1A#3) plants during aging. The seeds were aged under 42 °C and 80% RH for 0, 11, 14, and 17 d and then germinated at 30 °C in darkness. Error bars in C and D indicate ± SE (n = 3).