Table 1.

Affinity and kinetic measurements of ZAP70 on ITAM3 peptides by SPR at 37 ${}^{°}$C (N ≥ 3)

Peptide	KD (µM)	$k_{\text{on}}$ (µM–1 s–1)	koff (s–1)	${\text{t}}_{\text{1/2}}$ (s)	n
ITAM3 N	20.7 ± 2.7	0.265*	5.5 ± 0.46	0.126	4
ITAM3 C	7.52 ± 1.0	1.52*	11.4 ± 4.6	0.061	4
ITAM3 NC	0.0962 ± 0.024	1.89 ± 0.356	0.227 ± 0.036	3.05	6

*Values are estimated from measured K_D and k_off. Parameter values are means of fits to individual SPR experiments ± SEM. Numbers of SPR experiments for each condition are shown in the column labeled n.