Exosome‐like nanoparticles from Mulberry bark prevent DSS‐induced colitis via the AhR/COPS8 pathway

A

Immunofluorescence analysis of COPS8 protein expression with anti‐COPS8 antibody (red) and with DAPI (blue) in the duodenum, jejunum, ileum, and colon from Villin‐Cre and COPS8‐lox alleles expressing (COPS8^fl ^/ ^fl ) and knockout COPS8 in IEC (COPS8^ΔIEC ) mice. Scale bar, 100 μm, data are represented from seven biological replicates per genotype.

B, C

Immunoblot analysis of COPS8 protein expression in the liver, kidney, lung, and IECs isolated from ileum of COPS8^fl ^/ ^fl and COPS8^ΔIEC mice and analysis of the associated proteins (COPS5, COPS6, COPS7, Cullin (CUL) 1 and 3) in lysates prepared from isolated IECs from COPS8^fl ^/ ^fl and COPS8^ΔIEC mice. Data are represented from seven biological replicates per genotype.

D

Body size (representative pictures, left) and weight (right) of COPS8^fl ^/ ^fl and COPS8^ΔIEC mice at 4 weeks of age. Data are represented as mean ± SEM from seven biological replicates per genotype. *P < 0.05, NS—non‐significant using Student’s t‐test.

E

Length of small intestine and colon from COPS8^fl ^/ ^fl and COPS8^ΔIEC mice. Data are represented as mean ± SEM from seven biological replicates per genotype. **P < 0.01, NS—non‐significant using Student’s t‐test.

Figure EV2. Deletion of COP9/COP9 Constitutive Photomorphogenic Homolog Subunit 8 (COPS8) in intestinal epithelial cells (IEC) affects COP9 complex integrity.