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. 2022 Jan 7;23(3):e53365. doi: 10.15252/embr.202153365

Figure EV3. Paneth cell loss in COPS8ΔIEC mice.

Figure EV3

  1. Representative hematoxylin and eosin (HE) staining of duodenum and jejunum showing loss of Paneth cells (black arrow) in COPS8ΔIEC mice. Scale bar 50 μm, data are represented from seven biological replicates per genotype.
  2. The granule protein, lysozyme, was examined by immunofluorescence (Red) and counted in the duodenum and jejunum (located above dashed line) of Villin‐Cre and COPS8‐lox alleles expressing (COPS8fl / fl ) and COPS8ΔIEC mice. Scale bar 200 μm. Data are represented as mean ± SEM from seven biological replicates per genotype. **P < 0.01 using Student’s t‐test.
  3. Transmission electron microscopy (TEM) of crypts of COPS8fl / fl and COPS8ΔIEC mice. The base of the crypt in COPS8ΔIEC mice is occupied by poorly differentiated columnar epithelial cells that lack secretory granules, rudimentary electron‐dense granules (black arrows), microvilli (yellow arrows), and granules in the lumen (blue arrows). Scale bar 5 μm.
  4. Goblet cells from COPS8fl/fl and COPS8ΔIEC mice were stained with Alcian blue and counted in the ileum. Scale bar 50 μm. Data are represented as mean ± SEM from seven biological replicates per genotype. NS—statistically non‐significant using Student’s t‐test.
  5. The marker for enteroendocrine cells, chromogranin, was detected by immunofluorescence and counted in the duodenum and jejunum of COPS8fl / fl and COPS8ΔIEC mice. Scale bar 100 μm. Data are represented as mean ± SEM from seven biological replicates per genotype. **P < 0.01 using, NS—non‐significant using Student’s t‐test.