Figure 3. Known short-lived proteins were captured, and E3 ligases and substrate recognition subunits of E3 ligase complexes were enriched in short-lived proteins under translational inhibition.

(A) Known short-lived cell cycle inhibitors and activators were recapitulated. Replicates are shown individually in the heatmaps. Gray indicates no detection in the plex in the heatmaps. (B) Gene set enrichment analysis (GSEA). Degron-containing proteins were significantly enriched in proteins deemed short-lived. The gene set contains proteins that have at least one known degrons. Log₂ fold changes (8 hr vs 0 hr) were used in GSEA. Higher ranks indicate shorter half-lives. (C) InterPro categories enriched among short-lived proteins. DNA-binding proteins, E3 ubiquitin-protein ligases, and substrate recognition subunits of E3 ubiquitin ligase complexes were significantly enriched. (D) Subcellular components enriched among short-lived proteins. See also Figure S3.