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. Author manuscript; available in PMC: 2022 Nov 18.
Published in final edited form as: Mol Cell. 2021 Oct 8;81(22):4722–4735.e5. doi: 10.1016/j.molcel.2021.09.015

Figure 4. Substrate recognition subunits of E3 ubiquitin ligase complexes displayed faster degradation than core components under translational inhibition.

Figure 4.

About 90% of the E3 ubiquitin ligase complex subunits were found in this work. Core components of cullin-RING ubiquitin ligase complexes (A) and anaphase-promoting complex/cyclosome (B) were stable, and substrate recognition subunits were quickly degraded under 8 hr of translational arrest. The list of E3 ubiquitin ligase complex subunits was from Gene Ontology. Western blotting (C) shows that four adaptor or catalytic proteins (KLHL12, CCNF, CDC20, and ANAPC11) were short-lived, and five core component proteins (CUL3, CUL1, ANAPC1, CDC23, and CDC16) were stable in U2OS cells. See also Figure S4.