| Antibodies |
| Anti-ATRX antibody produced in rabbit |
Sigma-Aldrich |
HPA001906 |
| Monoclonal Anti-Vinculin antibody produced in mouse |
Sigma-Aldrich |
V9131 |
| p53 Antibody |
Cell Signaling Technology |
#9282 |
| Cyclin D1 (92G2) Rabbit mAb |
Cell Signaling Technology |
#2978 |
| Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse |
Sigma-Aldrich |
A8592 |
| Anti-β-Actin Antibody (C4) |
Santa Cruz Biotechnology |
sc-47778 |
| CUL3 Antibody |
Cell Signaling Technology |
#2759 |
| KLHL12 (2G2) Mouse mAb |
Cell Signaling Technology |
#9406 |
| CUL1 Antibody |
Cell Signaling Technology |
#4995 |
| Cyclin F (D9K2U) Rabbit mAb |
Cell Signaling Technology |
#81045 |
| APC1 (D1E9D) Rabbit mAb |
Cell Signaling Technology |
#13329 |
| APC8 (D5O2D) Rabbit mAb |
Cell Signaling Technology |
#15100 |
| APC6 (D8D8) Rabbit mAb |
Cell Signaling Technology |
#9499 |
| CDC20 (D6C2Q) Rabbit mAb |
Cell Signaling Technology |
#14866 |
| APC11 (D1E7Q) Rabbit mAb |
Cell Signaling Technology |
#14090 |
| ITCH (D8Q6D) Rabbit mAb |
Cell Signaling Technology |
#12117 |
| PJA2 Antibody |
Cell Signaling Technology |
#40180 |
| LRSAM1 (D1O5S) Rabbit mAb |
Cell Signaling Technology |
#28405 |
| RCHY1 Antibody |
Cell Signaling Technology |
#5754 |
| Critical commercial assays |
| TMTpro™ 16plex Label Reagent Set |
ThermoFisher Scientific |
A44520 |
| Deposited data |
| The mass spectrometry data have been deposited in the ProteomeXchange Consortium. |
This paper |
PXD024513 |
| Experimental models: Cell lines |
| U2OS |
ATCC |
HTB-96 |
| HEK293T |
ATCC |
CRL-3216 |
| HCT116 |
ATCC |
CCL-247 |
| RPE1 |
ATCC |
CRL-4000 |
| Oligonucleotides |
| siRNA Universal Negative Control #1 (siNC_1) |
Sigma-Aldrich |
SIC001 |
| siRNA Universal Negative Control #2 (siNC_2) |
Sigma-Aldrich |
SIC002 |
| ATRX, siRNA-1 (5’-3’) GGAUUCAACCUCUUGAGGA[dT][dT] UCCUCAAGAGGUUGAAUCC[dT][dT] |
Sigma-Aldrich |
SASI_Hs01_00081483 |
| ATRX, siRNA-2 (5’-3’) CUACAAUGAUGUAUUGACA[dT][dT] UGUCAAUACAUCAUUGUAG[dT][dT] |
Sigma-Aldrich |
SASI_ Hs02_00302645 |
| ATRX, siRNA-3 (5’-3’) CAGAUUCUACCAUGGUAGA[dT][dT] UCUACCAUGGUAGAAUCUG[dT][dT] |
Sigma-Aldrich |
SASI_ Hs02_00302642 |
| ATRX, siRNA-4 (5’-3’) CAAAGUGGCUCAUCAUCAU[dT][dT] AUGAUGAUGAGCCACUUUG[dT][dT] |
Sigma-Aldrich |
SASI_Hs02_00302643 |
| ATRX, siRNA-5 (5’-3’) CAUUGACUAUUACCGUUUA[dT][dT] UAAACGGUAAUAGUCAAUG[dT][dT] |
Sigma-Aldrich |
SASI_Hs01_00081480 |
| Software and algorithms |
| R package ClusterProfiler 3.16.1 |
(Yu et al., 2012) |
https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
|
| R package Limma 3.40.6 |
(Ritchie et al., 2015) |
https://bioconductor.org/packages/release/bioc/html/limma.html
|
| R package Peptides 2.4.2 |
(Daniel Osorio, 2015) |
https://cran.r-project.org/web/packages/Peptides/index.html
|
| R 4.0.2 |
N/A |
https://www.r-project.org/
|
