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Heme binding to COA7. (A) Difference absorption spectra of COA7 (1 μM) with increasing concentrations of Fe(III)-PPIX (from 0 to 10 μM) as indicated. (B) The binding curve was fitted to the data from A using an equation describing a single binding site (Y = Bmax * X/KD + X) with GraphPad Prism. (C) UV-Vis absorption spectra of COA7 in the presence of one molar equivalent of Fe(III)-PPIX (black); Fe(II)-PPIX (red), and Zn(II)-PPIX (dotted, black).
