Fig. 1.

N. brasiliensis smES products suppress OVA-induced allergic immune responses. (A) C57BL/6 mice were immunized with smES or control plus OVA in alum on days 0 and 14 and then challenged with the intranasal (i.n.) administration of OVA or PBS. Six days after the challenge, OVA-induced inflammation in the lung was analyzed. (B) Absolute numbers of eosinophils (Eos.), neutrophils (Neut.), lymphocytes (Lymph.), and macrophages (Mac.) in the BAL fluid are shown. These results were obtained using values from cell counting, the percentages of the cells, the total cell number per milliliter, and the volume of the BAL fluid recovered. Data from one experiment are representative of four independent experiments. (C and D) Lungs were fixed and stained with (C) H&E or (D) PAS. (Scale bars, 100 mm.) (E) Total RNA was prepared from the lung tissue, and the expression of Gob5, Muc5ac, and Muc5b was determined by quantitative RT-PCR. Relative intensities (Hprt; mean of three samples) are shown with SD. Mean values from four mice (B) per group are shown with SD. *P < 0.05, **P < 0.01.