TABLE 3.
PCR-based methods for detection of Cryptosporidium in stool
| Reference | Oocyst purification | Extraction method | Target |
|---|---|---|---|
| Laxer et al. (71) | Sucrose/Percoll centrifugation | Prot K/Ph/Chla | Anonymous (71) |
| Ranucci et al. (120) | Centrifugation | Boiling | COWP-190 |
| Webster et al. (148) | NaCl centrifugation | Prot K/Ph/Chl | Anonymous (148) |
| Awad-El-Kariem et al. (7) | NaCl centrifugation | Prot K/Ph/Chl | SSUc rRNA |
| Leng et al. (76) | None | Prot K/PCR spin column | Anonymous (71) |
| Morgan et al. (97) | None | Boiling | Anonymous (97) |
| Benigno Balabat et al. (8) | None | Prot K/Ph/Chl | Anonymous (71) |
| Gobet et al. (48) | NaCl centrifugation | Boiling | Anonymous (71) |
| Zhu et al. (154) | None | Glass beads/boiling/EtOH pptb | SSU rRNA |
a
Proteinase K digestion followed by phenol-chloroform extraction and ethanol precipitation.
b
Ethanol precipitation.
c
SSU, small subunit.