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. Author manuscript; available in PMC: 2022 Mar 3.
Published in final edited form as: J Vis Exp. 2021 Jul 30;(173):10.3791/62797. doi: 10.3791/62797

Figure 2 (modified from20): Nodal ligands promote C&E morphogenesis and germ layer formation in zebrafish blastoderm explants.

Figure 2 (modified from20):

(A) Diagram of injection and explantation of zebrafish embryos. (B-E) Representative bright-field images of live blastoderm explants of the indicated conditions/genotypes at the of equivalent the 2–4 somite stage. N = number of explants from two to four independent trials. (F) Time-lapse DIC series of a representative explant from a WT embryo injected with 10 pg ndr2 RNA. (G)Representative images of the whole-mount in situ hybridization for the transcripts indicated in explants from WT embryos injected with 10 pg ndr2 RNA. Scale bars are 200 μm.