Figure 5.

LDHA inhibition induces cell cycle arrest in G₂/M phase in cervical squamous cell carcinoma and endocervical adenocarcinoma cells. (A) Flow cytometric analysis was performed to detect proliferation of HeLa and SiHa cells following treatment with oxamate for 48 h. (B) Cell distribution analysis of each group. Western blot analysis was used to detect expression of Cyclin B1, Cyclin A, CDK1 and p21 in (C) HeLa and (D) SiHa cells following treatment with oxamate for 48 h. Western blot analysis was used to detect expression of Cyclin B1, Cyclin A, CDK1 and p21 in LDHA KD (E) HeLa and (F) SiHa cells. *P<0.05, **P<0.01 vs. con. Con, control; KD, knockdown; LDHA, lactate dehydrogenase A chain; CDK, cyclin-dependent kinase.