Figure 7.

LDHA inhibition induces cell cycle arrest and apoptosis via the JNK signaling pathway. Western blot analysis was used to detect expression of p38, JNK, p-p38 and p-JNK in HeLa cells following (A) treatment with oxamate for 48 h or (B) LDHA KD. *P<0.05, **P<0.01 vs. con. (C) Phosphorylation of JNK and (D) viability were analyzed following treatment with oxamate, SP600125 (10 µM) or oxamate + SP600125 in HeLa cells. **P<0.01 vs. oxamate. (E) Phosphorylation of JNK and (F) viability were analyzed in LDHA-knockdown HeLa cells following treatment with SP600125. **P<0.01 vs. KD. Con, control; KD, knockdown; LDHA, lactate dehydrogenase A chain; p-, phosphorylated.