Skip to main content
. 2021 Jun 3;373(6551):236–241. doi: 10.1126/science.abi5224

Fig. 3. Fe-S cluster sites in nsp12 are important for activity and interactions with nsp13.

Fig. 3

(A) RNA polymerase activity of anoxically purified RdRp (all lanes except Zn-nsp12) (RdRp at 1 μM) and of aerobically purified and Zn-reconstituted RdRp containing two zinc ions per protomer (three technical replicates are shown; n = 4). (B) Schematic of the complex required for coronaviral replication (10), in which the two Fe-S clusters and their coordination spheres are highlighted. ExoN, exoribonuclease; SSB, single-stranded DNA-binding protein; 2′-O MTase, 2′-O methyltransferase. (C) Co-IP of nsp12 WT or variants recombinantly expressed in Vero E6 cells cotransfected with helicase nsp13 and accessory factors nsp7 and nsp8 (Strep II tagged) probed with antibodies against FLAG, Strep II, or nsp13 (three technical replicates are shown; n = 4).